• 1.

    Can the Q-VOA assay be performed on a smaller volume of peripheral blood?

    Yes, the Q-VOA assay in the Persaud lab can be performed on a smaller volume of peripheral blood. In fact, the Persaud lab specialized in the Q-VOA assay on pediatric specimens which is done from a very small volume of peripheral blood. The smallest volume of blood that is accepted for pediatric studies is 2 milliliters.

  • 2.

    How many ddPCR samples have been performed at the Persaud lab?

    The Persaud lab has processed over 5,000 samples for ddPCR quantification (>30,000 individual reactions).

  • 3.

    How should dried blood spots (DBS) be stored?

    DBS should be dried overnight (>4 hours) before being stored. Each individual DBS card should be stored in a firmly zipped, air-impermeable bag (ex. Ziploc) with one desiccant. DBS can be stored and shipped at room temperature if sent within two weeks of collection. For long term storage, the ideal temperature would be in the freezer at -20/-80oC, although samples stored in the refrigerator at 4oC/Room Temperature may be accepted. Contact the Persaud lab for further details.